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Coordinator

Marianne DELARUE (UFR Sciences - Université Paris-Saclay).

Prerequisites

- Basic knowledge of cell functioning (division, expansion and differentiation) - To be able to describe the main developmental stages of angiosperms - To know the basics of gene expression and protein synthesis control.

Objective

• Describe and understand the molecular and cellular determinism of the main stages of angiosperms development
• To know the major regulators of developmental transitions and their signalling pathways, whether endogenous (hormones, peptide signals, etc...) or exogenous (light and photoperiod) and their possible interactions.
• To know and explain the main experimental methods and approaches used in developmental biology (direct genetic approaches, gene targeting and genome editing, protein-protein and protein-DNA interaction studies, spatio-temporal expression analysis, etc.)
• Know how to use databases and basic bioinformatics tools (sequence alignment, BLAST, Image J, etc…).
• To become aware of the so-called EVO-DEVO approaches and the challenges of translational biology in order to exploit knowledge acquired on models to cultivated species and agronomic interests.

Complementary skills

• To be able to analyze a scientific article
• To be able to present and synthesize the results of a nuclear immunolocalization test performed on different tissues.

Format

Alternating courses and/or conferences), interactive tutorials and practical work. Students will give two presentations in English: a presentation on an analytical method and a presentation of the results obtained at the end of the practical sessions. The visit of experimental cultures of an INRAE centre is also planned.

Bibliography

• BIOLOGIE VÉGÉTALE. Croissance et Développement. JF Morot-Gaudry, R. Prat. Editions Dunod. 2017 - PHYSIOLOGIE VEGETALE. M. Coupé et B. Touraine. Editions Ellipses. 2016
• BIOCHEMISTRY AND MOLECULAR BIOLOGY OF PLANTS. B. Buchanan, W. Gruissem, R. L. Jones. Editions Wiley Blackwell. 2015.

English
Elective TU (Choice between this TU "How to build a plant?" and the TU "Seeds: from biology to industry").
Lectures: 27h, Tutorials: 12.5h, Practicals: 5.5h, Distance learning: 5h
ECTS: 4
Location: Orsay